Prof. Dr. Mike Heilemann

Professor for Single Molecule Spectroscopy

Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Germany


Research Positions

Visiting Scientist, MBI NUS, Singapore

Guest Scientist, ALMF at EMBL, Heidelberg

Overseas Visiting Scholar, St John’s College, Cambridge University

Assistant Professor (W2) for Biophysics, University of Würzburg, Germany

Junior Group Leader, Physics Department, Bielefeld University, Germany

Postdoctoral fellow with Dr. A. Kapanidis, University of Oxford, UK


Honors and Awards

St. John’s Oversea Fellowship, University of Cambridge, UK

EPA-PPA paper prize, European Photochemistry Association

BMBF junior research group, FORSYS program (1.5 Mio€)

DAAD fellowship

Egide fellowship


Selected papers

1. Whole-cell, 3D and multicolor STED imaging with exchangeable fluorophores. Spahn C, Grimm JB, Lavis LD, Lampe M, Heilemann M*. Nano Letters 2019, 19, 500-505.

2. Correlative Single-Molecule FRET and DNA-PAINT Imaging Deussner-Helfmann NS, Auer A, Strauss MT, Malkusch S, Dietz MS, Barth HD, Jungmann R, Heilemann M*. Nano Letters 2018, 16, 4626-30.

3. Quantitative single-molecule imaging of TLR4 reveals ligand-specific receptor dimerization. Krüger CL, Zeuner MT, Cottrell GS, Widera D, Heilemann M*. Science Signaling 2017,10, eaan1308.

4. Linear ubiquitination of cytosolic Salmonella locally activates NF-?B and restricts bacterial proliferation. Van Wijk SJL, Fricke F, Herhaus L, Hötte K, Pampaloni F, Fulda S, Heilemann M*, Dikic I*. Nature Microbiology 2017, 8, 2:17066.

5. Temporal accumulation analysis provides simplified artifact-free analysis of membrane-protein nanoclusters. Spahn C, Herrmannsdörfer F, Kuner T, Heilemann M*. Nature Methods 2016, 13, 963-964.

6. A set of homo-oligomeric standards allows accurate protein counting. Finan K, Raulf A, Heilemann M*. Angewandte Chemie 2015, 54, 12049-52.

7. SLAP: Small Labelling Pair for Single-Molecule Super-Resolution Imaging. Wieneke R, Raulf A, Kollmansperger A, Heilemann M*, Tampé R*. Angewandte Chemie 2015, 54, 10216-9.

8. Arts and artifacts in single-molecule localization microscopy – beyond attractive images. Endesfelder U, Heilemann M*. Nature Methods 2014, 11(3), 235-8.

9. Super-resolution fluorescence imaging of chromosomal DNA using click chemistry. Zessin P, Finan K, Heilemann M*. Journal of Structural Biology 2012, 177, 344-348.

10. Monitoring multiple distances within a single molecule using switchable FRET. Uphoff S, Holden SJ, Le Rest L, Periz J, van de Linde S, Heilemann M, Kapanidis AN. Nature Methods 2010, 7, 831-6.