Project B2: Electron tomography of cell-cell junctions to address the Blood-Brain-Barrier using correlative light and electron microscopy methodologies

Amparo Acker-Palmer & Achilleas Frangakis

Work package 1: Visualization of cell-cell junctions using cryo-ET

Work package 2: Mechanism of BBB disruption in Reelin-deficient mice using cryo-ET

Figure: An example of a cryo-electron tomogram of a cell-cell junction.

Computational Biophysics: Ranking the Model Plethora

Can we discriminate different models using molecular dynamics simulations?

Figure: Molecular dynamics simulations

Figure: Molecular model of a cell-cell junction