Project A1: Light sheet-based fluorescence microscopy, sample holder systems, high-throughput/high-content LSFM
Ernst Stelzer
Work package 1: Meticulous description of the image recording process in mathematical, physical and engineering terms: Device-dependent metadata
Work package 2: Recording image data in event-driven adaptive feedback loops: Experiment-dependent metadata
Work package 3: Applications of event-driven adaptive feedback loops
Video: Lateral root development of a plant.
Video: Last seven days development of a cricket.
Video: Multi-dimensional data collection: three-dimensional imaging of multiple targets in three-dimensional specimens main-tained under near-natural conditions (nnc) as a function of time.
Figure: Automated Pipeline for Multiscale Quantitative Characterization. Multi-dimensional data collection: three-dimensional imaging of multiple targets in three-dimensional specimens main-tained under near-natural conditions (nnc) as a function of time.
multiple targets / time points / orientations, from 10s of Gbytes to Mbytes, features at multiple scales, single cell level
Figure: Six days: Life and Death of Murine Pancreas Organoids
LSFM-dedicated Sample Holders
Figure: Ultra-thin FEP-foil cuvettes in ZEISS Lightsheet Z.1 LSFM.
(a) General setup. (b) Microscope chamber with specimens in FEP-foil cuvette. (c) FEP-foil cuvette connected to sample holder. Approximately 2 x 2 x 10 mm3. FOV 0.8 x 0.8 x 1.6 mm3
Video: Imaging & Tracking of all Liver Organoids within a FEP-foil Cuvette. Time resolved 3D illustration of a growing liver organoid with cell segmentation and tracking.
Development of LSFM/DSLM
| CAD-DESIGN |  |
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| monolithic | double-sided illum/detection | inverted double-sided + CvFM | |
| Prototype |  |
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