Project A3: New image registration and reconstruction tools for molecular characterisation of lipid uptake mechanisms in Mycoplasma
This work aims to exhaustively explore the advantages of direct detectors for electron tomography and, in parallel, to compensate for the imperfection of the imaging system, thus improving the resolution of the cryo-electron tomograms.
Improve image quality by fully exploring the properties of the
Dose-fractionation movies (motion correct)
Local shifts (alignment)
Local CTF correction
Weighted-Backprojection, ART, SIRT and SART provide different solutions!
WBP reconstructs a flat power-spectrum
SART minimizes the difference between the micrograph p and the projected reconstruction f
Algebraic Reconstruction Methods allow for Local Nyquist-Limited Precise Alignment
Resolution Improvement in Cryo-ET by Advanced Local Alignment
Use of the SART to maximize the similarity between the projection of the reconstruction and the recorded data:
Goal: Provide completely undistorted reconstructions